The effect of anaerobic exercise with melatonin consumption on the expression of Bax and Bcl-2 markers in rat myocardium after ischemic-reperfusion

Background: The present research aims to examine the effect of anaerobic exercise with melatonin consumption on the expression of Bax and Bcl-2 markers in rat myocardium after ischemic-reperfusion by isoprenaline.

Methods: In the present experimental study, 28 male Wistar rats weighing approximately 200-250 g with two to three months old were divided into five groups: pilot (n=14), control (n=4), melatonin (n=4), anaerobic (n=4) and melatonin anaerobic (n=4). Pilot group were divided into two groups, isoperalin (n=7) and normal (n=7): isoperalin group injected isoprenaline with dose of 150 and 125 mg/kg BW with 24 hours in two consecutive days; and normal group has no injection. Then more fibros level was confirmed in isopernalin into normal groups used Masson-trichrom tanique. In the following Rats in melatonin group were gavaged every day for one month using a dose of 10 mg/kg BW. Meanwhile, rats in anaerobic group and melatonin anaerobic group were exposed training course with frequency of three times weekly for one month. But control group were injected only with isopernalin in the end of one month. Finally, rats were sacrificed after confirmation of infarct and expressions of bax and bcl2 gene were studied by real-time method.

Results: Melatonin treatment and anaerobic training have negligible effect on Bax and Bcl-2 gene expression. In the other hand, anaerobic exercise with consuming melatonin can decrease and increase Bax and Bcl-2 gene expression respectively and show significant effect, compared to treatment with melatonin alone.

Conclusion: The anaerobic exercise with consuming melatonin into consuming melatonin alone can reduce inactive induced-Infarction level.