INVESTIGATION OF BIOACTIVE CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF DIFFERENT FRACTIONS FROM HERNIARIA HEMISTEMON J.GAY

This study was carried out the phytochemical screening and evaluate antibacterial, antifungal and antioxidants effects of petroleum ether (40-60 °C), diethyl ether, chloroform, ethyl acetate, ethanol 96% and ethanol 70% fractions obtained from the aerial part of Herniaria hemostimon J. Gay. Phytochemical analysis showed the presence of terpenoids, steroids, flavonoids, alkaloids, tannins and saponins in different fractions the plant. Quantitative concentration of phenols, flavonoids, alkaloids, tannins and saponins were detected. HPLC analysis of H. hemostimon identified 21 flavonoids and 21 phenolic compounds. Successive fractionation has been carried out where Di-(2-ethylhexyl) phthalate (DEHP and protocatechuic acid were isolated and identified using FT-IR, 1H-NMR and MS spectroscopic. Furthermore, the antimicrobial activity of successive extract of H. hemistemon against 5 bacterial strains and 2 fungal and yeast strains carried out by the disc diffusion method. The diethyl ether and ethyl acetate fraction showed the highest activity against all the tested bacterial and fungal strains. The ethyl acetate fraction showed the highest antioxidant activity. This study suggested that the bioactivities of H. hemistemona can be used as a source of medicinal compounds due to there's significant antioxidant and antimicrobial activities.